Composite

Part:BBa_K3093002:Design

Designed by: Jin Qianwei   Group: iGEM19_ECUST_China   (2019-10-16)
Revision as of 14:19, 20 October 2019 by Still Water (Talk | contribs) (→‎Design Notes)

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Cellobiose operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of Pcel and chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR.

Source

E.coli K12 MG1655

References