Coding

Part:BBa_K2980012

Designed by: Zikang Huang   Group: iGEM19_Tsinghua   (2019-10-16)
Revision as of 11:03, 20 October 2019 by Billsfriend (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cry2-mCherry-Rluc

Rluc (Part:BBa_K1722005) was linked to the C terminal of Cry2-mCherry (Part:BBa_K2980006) and co-transformed with CIB1-GCN(4)-mEGFP-FUSLCD （Part:BBa_K2980009) to make it possible for Rluc to be recruited into phase. Then, as a control, we eliminated every component in both plasmid that may cause phase separation. Also, in order not to disturb the enzymatic activity, Rluc in both experimental group and control group were linked to mCherry. In detail, control group contained CIB1-mEGFP and mCherry-Rluc. We expected that after cultured in light, those two group would exhibit different enzymatic activity. Because phase could enrich both enzyme and substrate, bacteria in experimental group would perform higher catalytic activity compared to control group.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 393
    Illegal BglII site found at 852
    Illegal BamHI site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 629
    Illegal BsaI.rc site found at 38
    Illegal BsaI.rc site found at 2871
    Illegal SapI.rc site found at 146


[edit]
Categories
Parameters
None