Composite

Part:BBa_K3221200

Designed by: Yitao Ke   Group: iGEM19_SCU-China   (2019-10-04)
Revision as of 12:50, 15 October 2019 by Koursera (Talk | contribs)


Galactose-mediated induction system, yeGFP as a reporter

GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project. Then, we designed to culture transformed yeast and WT in YPD medium and YPG medium separately. Through detecting green fluorescence to verify the function of GAL4 promoter. T--SCU-China--scu-pGAldelay2019.png

Fig.1 Fluorescence observation A1 and B1: WT in YPG；A2 and B2 : Transformed in YPD；A3, A4, B3 and B4: Transformed in YPG. Visible Fluorescence in B3 and B4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


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