Regulatory
Part:BBa_K3239006
Designed by: Xingyu Zhou Group: iGEM19_ShanghaiFLS_China (2019-10-15)
yEGFP3
A yeast- and FACS-optimized GFP variant
Usage and Biology
This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.
Functional Parameters
yEGFP3 is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.
Reference
Protein: https://www.ncbi.nlm.nih.gov/protein/82569482
Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479
Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//classic/reporter
None |