Composite

Part:BBa_K3102024:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 09:50, 12 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)


T7-RBS-EG1-ter (secreted EG1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 362
    Illegal AgeI site found at 452
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EG1:Sequence synthesized and optimized for E.coli on Geneius

Source

Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

"Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene." Penttilae M., Lehtovaara P., Nevalainen H., Bhikhabhai R., Knowles J.K.C. Gene 45:253-263(1986)