Coding

Part:BBa_K3102000:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-02)
Revision as of 16:38, 12 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎References)


RibA (coding for GTP cyclohydrolase-2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 319
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal AgeI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331


References

"Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli." Foor F., Brown G.M. J. Biol. Chem. 250:3545-3551(1975)

Lin Z, Xu Z, Li Y, Wang Z, Chen T, Zhao X. Metabolic engineering of Escherichia coli for the production of riboflavin. Microb Cell Fact. 2014;13(1):1–12.

Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin, Microbial Cell Factories, 2014, 13:104.