Composite
N1

Part:BBa_K3308081

Designed by: Jemy Varghese, Harrison Green, Ripal Sheth, Victor So, Mel Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)
Revision as of 11:45, 14 October 2019 by Jvargh (Talk | contribs)


GB1-GTNPC-[NrdJ-1 N (1-4)]-[gp41-1 N]

N1 +MBP

File:T--Pittsburgh--nested inteins overview.png
Figure 1. Nested Intein design overview

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal BsaI.rc site found at 1227
    Illegal SapI.rc site found at 1527


[edit]
Categories
Parameters
None