Coding

Part:BBa_I746916:Experience

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-09-30)
Revision as of 09:40, 6 October 2019 by Andho863 (Talk | contribs) (Applications of BBa_I746916)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table.

Figure 1 present a 1 liter culture of induced E.coli BL21(DE3) CBD-sfGFP on a UV-table. The picture shows that an induced culture of CBD-sfGFP fluoresces at 302 nm.




















Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.




















User Reviews

UNIQ8e6e97fc1c6034e7-partinfo-00000000-QINU UNIQ8e6e97fc1c6034e7-partinfo-00000001-QINU