Coding

Part:BBa_K112002:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)
Revision as of 23:32, 3 September 2008 by Sixpi (Talk | contribs) (→‎Design Notes)

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T4 holin, without stop codon, berkeley standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

Source

References