Coding

Part:BBa_K112002:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)
Revision as of 23:28, 3 September 2008 by Sixpi (Talk | contribs) (→‎Design Notes)

T4 holin, without stop codon, berkeley standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112002

bx1 Forward biobricking of {T4 Holin>} ccagtGAATTCatgAGATCTatggcagcacctagaatatc bx4 Reverse biobricking of {T4 Holin>} cgttaGGATCCtttagcccttcctaatattctggcc

Source

References