Composite

Part:BBa_K2963014

Designed by: Experiment group advisor of JNU-China   Group: iGEM19_JNU-China   (2019-09-10)
Revision as of 16:03, 18 October 2019 by GHX (Talk | contribs)


BsracE18 - Bicistronic containing RBS18 to stabilize gene expression.


Usage and Biology

The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
    Illegal NheI site found at 999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None