Loop Assembly

As we prepare for iGEM 2019, I have been trying to decide how to support Type IIS assembly methods for the Registry and the 2019 iGEM Competition. Since [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks 2016], iGEM has allowed teams to submit parts in the PhytoBricks (Type IIS Golden Gate) standard , but have not fully supported any Type IIS standard.

Our current proposal is as follows:

iGEM fully adopts the Loop Assembly standard with Level 0 (basic) parts adhering to the MoClo and/or PhytoBricks Golden Gate assembly standards.

Adoption of Loop would include:

• giving teams full credit for submitting samples in this standard
• updating the Registry software to support the standard
• creating documentation for our users
• distributing a collection of basic parts and cloning vectors in the 2019 Distribution Kit.

iGEM would, of course, continue to support and send out BioBrick parts for the foreseeable future.


Before we finalize the plans for adopting Loop, I would like to collect input from our community. If you have any comments or thoughts on iGEM adopting and supporting Loop (including the MoClo and/or PhytoBricks assembly standards), please send an email to:

Why Loop Assembly

Adoption

We know several groups, including academic/research labs and industry, that are already using Loop Assembly. We want to ensure that the assembly method we support is widely used, tested, and has flexibility for different applications and groups.

Loop Assembly is compatible with a variety of Golden Gate standards for Level 0 parts, most importantly MoClo and PhytoBricks. Both of these standards are widely used, and MoClo's fusion sites completely overlap with PhytoBricks.

OpenMTA

Developed by OpenPlant and The BioBricks Foundation, the OpenMTA

Loop Assembly