Reporter

Part:BBa_K2611003:Design

Designed by: Qinling Qiu   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 06:52, 17 October 2018 by White (Talk | contribs) (→‎References)

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Spacer J23101-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
    Illegal NheI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer sequence and the NGG site should be closely to the promoter (no space in this part).


Source

The spacer sequence is generated by a special algorithm.[1] And the backbone we used is from BBa_K516132. [1]Didovyk A, Borek B, Hasty J, et al. Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9[J]. Acs Synthetic Biology, 2016, 5(1):81-88.

References

[1]Didovyk A, Borek B, Hasty J, et al. Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9[J]. Acs Synthetic Biology, 2016, 5(1):81-88.