Composite

Part:BBa_K2680117

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 14:19, 15 October 2018 by Jaurban (Talk | contribs)


3G pBad

pBAD promoter variant flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pBAD regulates the ara operon in bacteria. pBad is induced by arabinose and repressed by glucose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 201


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