RBS

Part:BBa_K2541101:Design

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)
Revision as of 05:22, 8 October 2018 by Shuting (Talk | contribs) (→‎Source)


Heat-repressible RNA-based thermosensor-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 31


Design Notes

When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.



Source

This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.

References

Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.