Coding
LaG2

Part:BBa_K2549002:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 02:07, 7 October 2018 by Wave (Talk | contribs)


anti-GFP (LaG2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LaG2 fuses two copies of LaG16 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP[1].

Source

IDT (gBlock)

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170