Composite

Part:BBa_K2598061:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-05)
Revision as of 11:14, 13 October 2018 by Gaoyuanli1998 (Talk | contribs)

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amilCP, amilGFP and eforred under resource allocator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 736
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

amilCP and eforred are reversed to avoid leakage. Strong terminators are added to avoid recombination.


Source

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)