Coding

Part:BBa_K2797003

Designed by: Matt Burridge, Kyle Stanforth, Sam Went   Group: iGEM18_Newcastle   (2018-09-28)
Revision as of 15:55, 2 October 2018 by Msburridge (Talk | contribs) (→‎Newcastle 2018 - Interlab Characterisation)


mNeonGreen

BBa_K2797003 (mNeonGreen) codon optimised for expression in E. Coli to replace mut3GFP as an improved fluorescent reporter in the interlab study.


Usage and Biology

The mNeonGreen protein is widely used in the imaging of cellular components due to it having a fluorescence 3-5 times that of GFP. Importantly however, it is thought to be more photostable than the mut3GFP used in the InterLab study, although there is little indication in the literature that it has been used as a reporter for the characterisation of circuits. Replacing mut3GFP with mNeonGreen allowed the investigation of whether the fast folding capabilities coupled with its brightness and higher photostability could yield a lower spread of fluorescence values in regard to the original mut3GFP, making it a better tool for part characterisation.


Newcastle 2018 - Interlab Characterisation

BBa K2797003.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]




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Categories
Parameters
None