Regulatory

Part:BBa_K2560007

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-08-20)
Revision as of 09:55, 30 September 2018 by DanielStukenberg (Talk | contribs)


Phytobrick version of BBa_J23100

Information category Part (marburg toolbox) in the right corner overview about the usage (marburg toolbox) characterisation parts (experiments etc.)



Figure 1. Relative promoter strenghts of promoters from the Anderson Collection in Vibrio natriegens. The promoter test construct is shown in Figure 2. Contruct were messured in quadruplicates and in three indipendent experiments.


Figure 2. Contruct for messuring promoter strenghts. The label "Tested Promoters" is a place holder for the respective promoter. The plasmid was assembled from eight basic parts by Golden Gate Assembly.



Usage and Biology

This is the Phytobrick version of the promoter BBa_J23100. Marburg 2018 characterized this part in Vibrio natriegens using the lux operon of Photorhabdus luminescens (BBa_K2560051). The messured relative promotor strenghts in Vibrio natriegens were correlated to the relative promotor strenghts in E. coli (iGEM Berkeley 2006). The parts sequence was verified by Sanger sequencing.


Plasmid map of the test construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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