Part:BBa_K2656013

sfGFP Coding Sequence

Part BBa_I746916 standarized as Phytobrick. Super Folder Green Fluorescent Protein coding sequence. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.

We have obtained the emission and excitation spectra of this protein following this protocol (REF) and with the parameters of the table 1 we have obtained the results of the graphic 1:

Parameter	Value
Sample number	0
Minimum excitation wavelenght (nm)	0
Maximum excitation wavelenght (nm)	0
Emission wavelenght (nm)	0
Minimum emission wavelenght (nm)	0
Maximum emission wavelenght (nm)	0
Excitation wavelenght (nm)	0
Gain (G)	0
Table 1. Parameters used to obtain the spectra

Graphic 1. sfGFP emission and excitation spectra

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 14