Part:BBa_K2632002

Promoter sifA

Gene sifA is a key gene located in Salmonella Salmonella pathogenicity island and has the function of maintaining the integrity of Salmonella-Containing Vacuole (SCV) and was reported that it expresses after infection. A fragment including sifA promoter region and its’s RBS region was amplified from Salmonella SL1344 genome.

Notice

This part carried it's native RBS. You can attach a CDS to it.
This part should be in a low copy plasmid such as PSB4K5,or it will express before Salmonella's entry to cell.

Use in Salmonella only.

Reference

1. Garmendia, J., Beuzón, C. R., Ruiz-Albert, J. & Holden, D. W. The roles of SsrA-SsrB and OmpR-EnvZ in the regulation of genes encoding the Salmonella typhimurium SPI-2 type III secretion system. Microbiology 149, 2385–2396 (2003). 3. Beuzon, C. R. Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. EMBO J. 19, 3235–3249 (2000). 4. Malik-Kale, P. et al. Salmonella- at home in the host cell. Front. Microbiol.2, 1–9 (2011) 5. Steele-Mortimer, O. The Salmonella-containing vacuole-Moving with the times. Curr. Opin. Microbiol. 11, 38–45 (2008)(2008).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]