Composite

Part:BBa_K2638022

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)
Revision as of 04:14, 3 September 2018 by Eschneider (Talk | contribs)

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Moderate constitutive expression system for OprC and CopD

Weak constitutive expression of OprC (BBa_K2638200) and CopD (BBa_K2638002) with weak anderson promoters J23107 and RBSs BBa_B0034 and a double terminator (BBa_K823017) to seperate them.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1091
    Illegal NheI site found at 1114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1397
    Illegal NgoMIV site found at 1728
    Illegal NgoMIV site found at 1932
    Illegal NgoMIV site found at 2976
    Illegal NgoMIV site found at 3177
    Illegal AgeI site found at 1391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 326


[edit]
Categories
Parameters
None