Collections/Cell-Free TX-TL
This year our team (Lethbridge Collegiate) has put all the protein components of a cell-free transcription-translation (TX-TL) system in BioBrick standard with affinity purification tags. The idea is to provide an easy to express and purify TX-TL system that anyone can perform and use for protein synthesis testing or teaching.
We sought to make all the TX-TL components available to all iGEM teams so anyone could easily produce this system for cell-free protein expression. In total there are 38 TX-TL proteins that we would like to register as a parts collection.
This collection will grow as we continue to develop a tRNA and ribosome purification strategy in BioBrick standard as well.
| Name | Description | Type | Created by | length | uses | seq |
|---|---|---|---|---|---|---|
| BBa_K2109108 | MS2 6XHis (V29I/d1FG) | Composite | Andy Hudson | 640 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443000 | AlaRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2862 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443001 | ArgRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1965 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443002 | AsnRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1632 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443003 | AspRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2004 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443004 | CysRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1617 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443005 | GlnRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1899 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443006 | GluRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1650 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443007 | GlyRS Alpha subunit optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1143 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443008 | GlyRS Beta subunit optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2301 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443009 | HisRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1506 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443010 | IleRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 3048 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443011 | LeuRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2814 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443012 | LysRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1750 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443013 | MetRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2265 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443014 | PheRS Alpha subunit optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1215 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443015 | PheRS Beta subunit optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2619 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443016 | ProRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1950 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443017 | SerRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1524 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443018 | ThrRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2160 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443019 | TrpRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1236 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443020 | TyrRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1506 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443021 | ValRS optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 3087 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443022 | MTF optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1179 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443023 | IF1 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 456 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443024 | IF2 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2904 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443025 | IF3 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 774 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443026 | EF-G optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2346 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443027 | EF-Tu optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1419 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443028 | EF-Ts optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1083 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443029 | RF1 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1314 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443030 | RF2 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1329 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443031 | RF3 optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1821 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443032 | RRF optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 789 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443033 | MK optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 879 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443034 | CK optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 1377 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443035 | NDK optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 717 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443036 | PPiase optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 822 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443037 | T7 RNA Polymerase optimized for expression in E. coli | Composite | Lethbridge iGEM 2017 | 2886 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443038 | tRNAPhe-MS2 | Composite | Lethbridge iGEM 2017 | 300 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2443039 | EYFP-Spinach transcription and translation (TX-TL) measurement device | Measurement | Lethbridge iGEM 2017 | 1063 | . . . caccttcgggtgggcctttctgcgtttata | |
| BBa_K2722000 | Exonuclease V, Subunit RecC | Coding | Enik Baligcs | 3369 | . . . ttaccgctgtttcgctttaatcagtcatga | |
| BBa_K2722001 | Exonuclease V, Subunit RecD | Coding | Enik Baligcs | 1827 | . . . ctggcggcgttgtttagttcgcgggaataa | |
| BBa_K2722002 | 6 times Chi recombination hotspot to inhibit Exonuclease V (RecBCD) activity | DNA | Enik Baligcs | 92 | . . . gccactgctggtggccactgctggtggcca |
- To add to this list, please use the category //collections/cell-free_tx-tl