Part:BBa_K2505005

(tra box)7-CMVmin-atipt4-IVS-IRES-log1-polyA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1442
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 26
  Illegal BglII site found at 72
  Illegal BglII site found at 118
  Illegal BglII site found at 164
  Illegal BglII site found at 210
  Illegal BglII site found at 256
  Illegal BglII site found at 302
  Illegal BamHI site found at 476
  Illegal XhoI site found at 2728
  Illegal XhoI site found at 2740
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1220
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1362
  

These two genes are derived from Arabidopais thaliana and encode enzymes necessary for synthesizing iP (isopentenyladenine) in mammalian cells. iP is a kind of cytokinins that are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. When these genes are introduced to human cells, EA.hy926, the cells produce iP heterologously. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form. LOG1 has the phosphoribohydrolase activity and converts inactive cytokinin nucleotides to the biologically active free-base forms.

The expression of these genes are induced by TraR protein.

The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage.

CMVmin (minimal CMV promoter: BBa_553022).