Regulatory
pT7

Part:BBa_I719005

Designed by: Imperial 2007   Group: iGEM07_Imperial   (2007-10-24)
Revision as of 05:19, 26 October 2007 by Ben Yi (Talk | contribs)

T7 Promoter

Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present. This part is identical to the part BBa_R0085 which currently doesn't exist.

This part has been characterized for temperature sensitivity in the Cell-Free Chassis by iGEM Imperial 2007. For more detail, please check the testing [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.9 results].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/bacteriophage/T7
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
//chassis/bacteriophage/T7
Parameters
negative_regulators
positive_regulators