Part:BBa_K2368007

Introduction

P_{Gal 1/10}-T1R2-CYC1t

This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator CYC1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor.

Fig.1 The schematic diagram of Gal1/Gal10+T1R2+CYC1

Design

We construct the part by Overlap Extension PCR. The Gal1/Gal10 promoter and CYC1 terminator stem from the PESC-Ura plasmid. We synthesize T1R2 by oligonucleotide synthesis.

Experiment

This part is used to express the sweetness receptor T1R2 in our experiment.

Fig.2 Electrophoresis of Gal1/Gal10+T1R2+CYC1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 563
  Illegal BglII site found at 2061
  Illegal BglII site found at 2481
  Illegal BglII site found at 2643
  Illegal BamHI site found at 674
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 291
  Illegal AgeI site found at 1572
  Illegal AgeI site found at 2790
  
• 1000
  COMPATIBLE WITH RFC[1000]