Composite

Part:BBa_K2250004

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-19)
Revision as of 08:11, 19 October 2017 by Shuailin10 (Talk | contribs)


Pconst-luxR-Plux-LacI-PlacI-CmR

This is the reception part of warrior 1/beggar 1/farmer 1 in our project. This part make the warrior 1/beggar 1/farmer 1 able to recept the 3OC6HSL secreted by warrior 2 so that the warrior 2 can kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


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