Reporter
GG98
Part:BBa_K2145105:Experience
Designed by: Laura Xinyu Chen, Katie Maruna, Melody Wu Group: iGEM16_Alverno_CA (2016-10-14)
Applications of BBa_K2145105
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG98 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
User Reviews
UNIQ5d0dbf52f8721316-partinfo-00000000-QINU UNIQ5d0dbf52f8721316-partinfo-00000001-QINU