Composite

Part:BBa_K1893000:Experience

Designed by: Alyssa Henderson   Group: iGEM16_Imperial_College   (2016-10-14)
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Applications of BBa_K1893000

We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.

This part worked as expected, and when we placed GFP downstream of the LasR promoter, we saw fluorescence when we induced with 3O-C12 AHL.

User Reviews

UNIQ7833e2688b6346a7-partinfo-00000000-QINU UNIQ7833e2688b6346a7-partinfo-00000001-QINU