Regulatory
pBAD

Part:BBa_K1893015:Design

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 12:48, 23 October 2016 by HOLL579 (Talk | contribs) (→‎Design Notes)


Arabinose inducible promoter (pBAD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098


===Design Notes===.

The reverse terminator BBa_B0025 (link) was added to part BBa_K1321333, for use in the pSB1A2 plasmid. This was done because a reverse terminator is present in the pSB1C3 plasmid, but not in pSB1A2

Source

This part was created by ligating the araC and pBAD inducible promoter construct in the distribution kit (BBa_K1321333) with the BBa_B0025 reverse terminator

References