RBS

Part:BBa_B0034

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.   Group: Antiquity   (2003-01-31)
Revision as of 09:15, 19 October 2016 by Areiss (Talk | contribs) (→‎Usage and Biology)

RBS (Elowitz 1999) -- defines RBS efficiency

RBS based on Elowitz repressilator.

Usage and Biology

William and Mary iGEM 2016

T--William_and_Mary--RBS_Characterization_Curves.png

We characterized the Anderson RBS library to better understand the strengths of each RBS under different induction conditions

IIT Madras 2016's Characterization

Characterization of popular BioBrick RBSs

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]

Experimentation

Biobrick RBSs B0030, B0031, B0032, B0034 were used in our 'Noise in Device' experiment to understand the role of RBS parts in giving rise to noise.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

biology-NA-
efficiency1

Note: The Elowitz RBS is the definition of efficiency 1.0.

Team Warsaw 2010's measurement

definition of 100% strength in our measurement.

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/coliroid
//rbs/prokaryote/constitutive/community
//regulation/constitutive
//ribosome/prokaryote/ecoli
Parameters
biology
efficiency1