Part:BBa_K2065002

T14-3-3 (S71L) - T14-3-3

Sequence

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1234
  Illegal EcoRI site found at 1389
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1234
  Illegal EcoRI site found at 1389
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1234
  Illegal EcoRI site found at 1389
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1234
  Illegal EcoRI site found at 1389
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1234
  Illegal EcoRI site found at 1389
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 937
  

LargeBiT-GGS10-CT52 Statistics

Protein Specifications
General Information	Number of amino acids		514
	Molecular weight		56797.79
	Theoretical pi		4.96
	Extinction coefficient		55030 of 54780
	Formula		C2480H3950N680O808S18
	Total numbers of atons		7936
Amino Acid Composition	Amino Acid	Frequency	Percentage(%)
	Ala(A)	53	10.3
	Arg(R	28	5.4
	Asn(N)	22	4.3
	Asp(D)	22	4.3
	Cys(C)	4	0.8
	Gln(Q)	12	2.3
	Glu(E)	60	11.7
	Gly(G)	39	7.6
	His(H)	6	1.2
	Ile(I)	28	5.4
	Leu(L)	53	10.3
	Lys(K)	32	6.2
	Met(M)	14	2.7
	Phe(F)	12	2.3
	Pro(P)	10	1.9
	Ser(S)	49	9.5
	Thr(T)	24	4.7
	Trp(W)	4	0.8
	Tyr(T)	22	4.3
	Val(V)	20	3.9
	Pyl(O)	0	0.0
	Sec(U)	0	0.0