Translational_Unit

Part:BBa_K2042010:Design

Designed by: Jonathan Tellechea   Group: iGEM16_Evry   (2016-09-02)
Revision as of 21:19, 15 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)

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RBS + Pct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1094
    Illegal BsaI site found at 1565


Design Notes

We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.

Source

The RBS were synthesized from the genomic sequence of P.putida, Pct gene were synthetised from genomic sequence of Clostridum propionicum.

References