Part:BBa_K1936001
Tom5-eGFP-LOV2
ALERT !!!Under construction !!
Usage
While LOV2 domain was previously described for optogenetical control of gene expression (BBa_K1742000) and promotor characterization (BBa_K660004), we took a new approach. The described fusion protein Tom5-eGFP-LOV2 is designed to recruit proteins of choice to the outer mitochondrial membrane (OMM) in engineered eukaryotic cells.
Tom5 is a part of the eukaryotic translocase of outer membrane protein complex and has a single membrane anchor unit that integrates into OMM. [1] We fused it to the N-terminal end of LOV2 domain from A.sativa. Upon exposure to blue light (473nm) the LOV2 peptide undergoes a conformational change and exposes its C-terminal J-alpha-helix which is then able to bind a specifically engineered PDZ domain (BBa_K1470005). [2]
Our blue light dependent system can be used to recruit any protein of interest to the outer mitochondrial membrane by simply fusing it to an ePDZ domain.
- Figure 2: The LOV2-based optogenetic switch is activated by blue light.
Biology
Characterization
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 33
Illegal BamHI site found at 712
Illegal XhoI site found at 515 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1226
//chassis/eukaryote/yeast
//function/sensor/light
//proteindomain/localization
//proteindomain/transmembrane
chassis | H.sapiens |
excitation | blue light |
function | mitochondrial recruitment |