Composite
Part:BBa_K1897025
Designed by: Keshiniy Madivannan Group: iGEM16_NUS_Singapore (2016-10-11)
J23100-34-ALR-Terminator
The construct contains a high expression promoter J23100 in a cassette (Part:BBa_K314100) with a strong RBS (Part:BBa_B0034), followed by a CDS of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493
Illegal NheI site found at 855 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 779
Illegal BamHI site found at 1481 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal AgeI site found at 897
Illegal AgeI site found at 1197 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 654
[edit]
Categories
Parameters
None |