Coding

Part:BBa_K1969001:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-05)
Revision as of 02:15, 27 October 2016 by StevenLai (Talk | contribs)

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Homo sapiens adrenoceptor beta 2 (ADRB2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


Design Notes

First, it has no obvious tag for researchers to detect it directly, so we further add a 6xhis tag at its C terminus. Second, as the N terminus sequence may decide the membrane localization in different host cells, we used the C terminus sequence of GPCR RI7 and STE2 which were both reported to be successfully located to yeast membrane to replace the corresponding sequence of ADRB2.


Source

Its from human genomic sequence. Because the original sequence is not BioBrick compatible, we use site mutation to eliminate the illegal PstI site by changing G at 192 bp to A.

References

The original biobrick part is BBa_K209472, because the original sequence is not BioBrick compatible, we use site mutation to eliminate the illegal PstI site by changing G at 192 bp to A.