Device

Part:BBa_K2088010

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-08)
Revision as of 17:28, 10 October 2016 by Summerice (Talk | contribs)


A device that expresses the XylE- catechol 2,3-dioxygenase to degrade the catechol .

Before the XylE - catechol 2,3-dioxygenase, the promoter J23100 and the RBS B0034 are connected. The device can be used to degrade the catechol in some degradation system to decrease the content of the catechol for the catechol can restrain the growth of the bacteria, also, the catechol is a kind of common degradation intermediate product.

Verification Test

  • We verified the function of xylE by constructing a device BBa_K2088010.
Figure 8: We construced a device that includes J23100, B0034, xylE and B0014. Transform the device into E.coli and culture at 37℃ overnight, the colonies showed a common white colour.
Figure 9: Drip 0.2mol/L catechol onto the colonies, the cells became bright yellow after 10 minutes. This indicates the xylE converts catechol to 2-hydroxy-cis,cis-muconic semialdehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 572
    Illegal AgeI site found at 923
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation
Parameters
device_type