Composite

Part:BBa_K1949032

Designed by: Yoshio Takata   Group: iGEM16_Tokyo_Tech   (2016-10-06)
Revision as of 07:01, 12 October 2016 by Kazuki (Talk | contribs)


PBAD-rbs-yafO

This part consists of Plac(BBa_R0010) + RBS(BBa_B0034) + yafO(BBa_K1949030).

toxin-antitoxin (TA) systems are cassettes consisting of stable protein, toxin which function as mRNA interferase and unstable protein, antitoxin which destroys function of toxin. Many TA systems exist in the E.coli genome. They can inhibit cell growth or induce cell killing. yafO is one of the toxin and is co-expressed with yafN. 　


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None