Part:BBa_K1555000:Experience

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg/L copper ion 2h 1mg/L copper ion

User Reviews

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••••• HSiTAIWAN

Detecting Copper in Chinese medicine Objective      Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine. Methods      Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).      Fluorescence was measured at: Excitation peak: 470nm Emission peak: 508nm every 30 minutes. Results      This part is still specific to copper in Chinese medicine, so it be apply in Chinese medicine examination. Discussion      Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of arsenic.

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This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter. |width='80%' valign='top'|

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