Reporter

Part:BBa_K2151008

Designed by: Matej Vucak, Katy Baker, James Provan   Group: iGEM16_Glasgow   (2016-08-30)
Revision as of 21:41, 8 October 2016 by Mvucak (Talk | contribs) (Added long description using main body.)


p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

This part consists of I13500 (GFP with strong ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720


[edit]
Categories
Parameters
None