RFC25 "Freiburg standard" parts
Contents
Reptorter proteins
Fluorescent proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Fluorescent proteins | |||
|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |
| Firefly luciferase | 61 | ||
| Renilla luciferase | 36 | ||
| NanoLuc luciferase | BBa_K1159001 | 19 | |
| Gaussia luciferase | |||