Regulatory

Part:BBa_K1840001:Design

Designed by: Julia Anna Adrian   Group: iGEM15_NTNU_Trondheim   (2015-09-18)
Revision as of 07:50, 18 September 2015 by Julia.A Adrian (Talk | contribs) (→‎References)

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Kgu promotor from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the kgu gene. We assumed it starts directly after the previous gene. This gene codes for PtxS, the repressor.


Source

This part was designed after information from the paper by DADDAOUA, A., KRELL, T., ALFONSO, C., MOREL, B. & RAMOS, J.-L. 2010. Compartmentalized Glucose Metabolism in Pseudomonas putida Is Controlled by the PtxS Repressor. Journal of Bacteriology, 192, 4357-4366.

References

DADDAOUA, A., KRELL, T., ALFONSO, C., MOREL, B. & RAMOS, J.-L. 2010. Compartmentalized Glucose Metabolism in Pseudomonas putida Is Controlled by the PtxS Repressor. Journal of Bacteriology, 192, 4357-4366