Coding
AvOprF

Part:BBa_K1648045:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-21)
Revision as of 08:21, 17 September 2015 by Alyssaliu (Talk | contribs) (→‎Design Notes)

AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with Azotobacter vinelandii as template using the following primers:

Forward primer: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAACTGAAAAACACCTTGGGCG

Reverse primer: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTGGGCTTGGGCTTCCAC

Insert oprF PCR product into psB1C3

Source

BBa_K1314016

References