Coding

Part:BBa_K1675001

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)
Revision as of 17:40, 18 September 2015 by JIAQIXU (Talk | contribs)

NhaB; sodium-proton antiporter; membrane protein

The gene, NhaB, is the archetypal sodium/proton anti-porter in E. coli. It is crucial when the NhaA's activity is limited, when NhaA is not sufficiently induced, and/or when NhaA is not activated. NhaA catalyzes the exchange of 3 protons per 2 sodium ions. Therefore, NhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1003
    Illegal AgeI site found at 1315
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli str. K-12 substr.
proteinNhaB