Coding

Part:BBa_K1675000

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)
Revision as of 13:16, 15 September 2015 by JIAQIXU (Talk | contribs)

glutamate decarboxylase A （GadA）

The gene, nhaA, is the archetypal Na+/H+ anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli str. K-12 substr.
proteinNhaA