Coding
DspBx
Part:BBa_K1659210:Design
Designed by: Wei Chung Kong Group: iGEM15_Oxford (2015-08-28)
Revision as of 17:09, 13 September 2015 by Weikongquee (Talk | contribs)
Dispersin B with mutation at nucleotide 582 (T to C)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 262
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 418
Design Notes and Sources
This part is a modified version of BBa_K1659200, where thymine 582 was mutated into a cytosine, conserving the His-194 which it encodes for. The site-directed mutagenesis was performed using a modified NEB Q5 Mutagenesis Protocol, which is fully described in our team's [http://2015.igem.org/Team:Oxford/Protocols Protocols Page].
References
[1] SnapGene software (from GSL Biotech; available at snapgene.com)