Reporter
mCherry

Part:BBa_J06504

Designed by: ytwang   Group: iGEM2005   (2005-07-18)
Revision as of 15:11, 28 March 2007 by Scmohr (Talk | contribs)

monomeric RFP optimized for bacteria

mRFP1-derived, altered to be a BioBrick by removing a PstI site and adding BioBrick ends. [mRFP1 was itself a derived from DsRed (via 33 mutations!)]

mCherry is one of several "second-generation" monomeric fluorescent proteins developed in Roger Tsien's laboratory at UCSD (cf., Nature Biotechnology 22, 1567 - 1572 (2004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//function/reporter/fluorescence
Parameters
abs --
biology
emission610
emit610
excitation587
excite587
lum --
proteinmCherry2
tagNone