Part:BBa_K1648050

PfOprF

OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode.

BBa_K1172501 is a biobrick which is a porin protein OprF from Pseudomonas fluorescens invented by iGEM2013 Germany Bielefeld team. and BBa_K1648050 is the construct including tetRpro(J13002) and BBa_K1172501 which is needed for the ongoing experiment.

The ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 844
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1236
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 757