Composite

Part:BBa_K1413023:Design

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 21:55, 1 November 2014 by Laurabibi (Talk | contribs) (→‎References)

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Promoter-RBS-bphr2 mutated-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352


Design Notes

Between each part, there is the scar SpeI/XbaI.

Source

The constitutuve promoter (BBa_J23114), RBS (BBa_B0034) and terminator (BBa_B0015) come from the 2014 kit distribution. bphr2 has been synthetized (it is the part BBa_K1413021).

References

Kensuke Furukawa and Hidehiko Fujihara, Microbial Degradation of Polychlorinated Biphenyls : Biochemical and Molecular Features, 2008. Journal of Bioscience and Bioengineering, Vol. 105, No. 5, 433–449.