Part:BBa_K1418061

phaCAB-p-GFPPhaC-B0015

Inducible PHB generator with GFP fused to PhaC (PHB synthase).

Schematic for functionalization of PHB granules. PhaC remains covalently bound to the PHB granule after polymerization and GFP can be fused to the N-terminal of PhaC, thus functionalizing the PHB granule. Direct production of PHB with the phaABC operon and expression of the GFP-PhaC fusion protein are both under lactose control.

A.) Nile Blue staining and B.) GFP expression of E. coli cells containing https://parts.igem.org/Part:BBa_K1418061. Microscope images of GFP bound PHB granules in E. coli after 24 h of growth with IPTG induction. White bar represents 5 µm.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4446
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 916
  Illegal BglII site found at 1741
  Illegal BglII site found at 5761
  Illegal BamHI site found at 4965
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 222
  Illegal NgoMIV site found at 293
  Illegal NgoMIV site found at 893
  Illegal NgoMIV site found at 1205
  Illegal NgoMIV site found at 1484
  Illegal NgoMIV site found at 2136
  Illegal NgoMIV site found at 2158
  Illegal AgeI site found at 5460
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4002